Two membrane-associated regions within the Nodamura virus RNA-dependent RNA
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Two membrane-associated regions within the Nodamura virus RNA-dependent RNA 1 polymerase are critical for both mitochondrial localization and RNA replication 2 3 4 Vincent U. Gant, Jr., Stephanie Moreno, Armando Varela-Ramirez, and Kyle L. 5 Johnson 6 7 Border Biomedical Research Center and Department of Biological Sciences, The 8 University of Texas at El Paso, 500 West University Avenue, El Paso, Texas, USA 9 10 11 Running title: Localization and membrane association of NoV RdRp 12 13 14 #Address correspondence to Kyle L. Johnson, [email protected]. 15 16 17 Word count for abstract: 194 18 19 Word count for importance section: 150 20 21 Word count for text: 8,227 22 JVI Accepts, published online ahead of print on 2 April 2014 J. Virol. doi:10.1128/JVI.03032-13 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
Two membrane-associated regions within the Nodamura virus RNA-dependent RNA polymerase are critical for both mitochondrial localization and RNA replication.
UNLABELLED Viruses with positive-strand RNA genomes amplify their genomes in replication complexes associated with cellular membranes. Little is known about the mechanism of replication complex formation in cells infected with Nodamura virus. This virus is unique in its ability to lethally infect both mammals and insects. In mice and in larvae of the greater wax moth (Galleria mellonella), Noda...
متن کاملTwo membrane - associated regions within the Nodamura virus RNA - dependent RNA 1 polymerase are critical for both mitochondrial localization and RNA replication
Two membrane-associated regions within the Nodamura virus RNA-dependent RNA 1 polymerase are critical for both mitochondrial localization and RNA replication 2 3 4 Vincent U. Gant, Jr., Stephanie Moreno, Armando Varela-Ramirez, and Kyle L. 5 Johnson 6 7 Border Biomedical Research Center and Department of Biological Sciences, The 8 University of Texas at El Paso, 500 West University Avenue, El P...
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Nodamura virus (NoV) and Flock House virus (FHV) are members of the family Nodaviridae. The nodavirus genome is composed of two positive-sense RNA segments: RNA1 encodes the viral RNA-dependent RNA polymerase and RNA2 encodes the capsid protein precursor. A small subgenomic RNA3, which encodes nonstructural proteins B1 and B2, is transcribed from RNA1 during RNA replication. Previously, FHV was...
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متن کاملFurther physicochemical characterization of Nodamura virus. Evidence that the divided genome occurs in a single component.
Nodamura virus, a small non-enveloped RNA virus, contains two species of RNA sedimenting at 22S (RNA-1) and 15S (RNA-2), a single major polypeptide of mol. wt. 40 X 10(3) and two minor polypeptides, of mol. wt. 38 and 43 X 10(3). Evidence is presented that the two RNA species are in the same particle. Although extraction of the virus with SDS-phenol yields the two species of RNA as separate ent...
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تاریخ انتشار 2014